Jacobs Journal of Veterinary Science and Research
Articles in Press
Volume 1 Issue 4
Effect of Gelatin Hydrogel Sponge Loaded with Bone Marrow Mesenchymal Stem Cells and Hepatocyte Growth Factor on Peripheral Nerve Regeneration
N. Sasaki*, J.P. Seo, H. Uchiyama, S. Haneda, K. Yamada, H. Furuoka, M. Itho, M. Yanagawa, Y. Tabata
Hepatocyte growth factor (HGF) can promote nerve cell proliferation. Gelatin hydrogel sponge can act as a scaffold for drug controlled release. . This study aimed to evaluate the beneficial effects of a gelatin hydrogel scaffold (sponge) loaded with BM-MSCs and HGF on radial nerve regeneration in a defect model. Twelve healthy calves (weighing 48.7 ± 5.54kg, mean ± S.D.) were used in this study. Stem cells were derived from bone marrow of ilium and cultured into culture dishes for two weeks. 1cm of the left radial nerve was defected under inhalation anesthesia.
Cyclical Shedding and Sustained anti-Phase 1 Antibody Responses in a Goat Herd Naturally Infected with Coxiella burnetii
Alicia D. Anderson*, Tahnee J. Szymanski, Kelly A. Fitzpatrick, Joshua S. Self, Rachael A. Priestley, William L. Nicholson, Gilbert J. Kersh
Coxiella burnetii is a worldwide zoonotic pathogen that can infect a variety of species and can cause Q fever in humans. Although an infected goat herd is a risk to human health, the duration of shedding and antibody responses in infected goats have not been clearly defined. In 2011, an outbreak of human Q fever in Washington and Montana was associated with infected goats, and one herd offered an opportunity for longitudinal sampling to investigate antibody responses and shedding of C. burnetii.
Puppy Morbidity and Mortality among Breeding Kennels in Nairobi, Kenya
Andrew Matole Konde*, George Karuoya Gitau, Japheth Kiptoon and Daniel Gakuya
The dog to human population ratio is estimated to range from 1:4 - 1:11. The human population of Kenya was estimated to be slightly over 43 million in 2012. Using the dog human population ratio range of 1:8 – 1:11, Kenya today has an estimated dog population of between 3.6 and 4.8 million. Dog breeders in Kenya are in the business to produce show, companion or guard dogs with an observed stillbirth rate of about 2.3 %, pre-weaning mortality of 11.4 % and a pre-weaning culling rate of 0.9 % for German shepherd dog.
Is Sperm Hyaluronidase Indispensable in Mammalian Fertilization?
Fertilization involves several precisely coordinated steps, including sperm dispersal of the oocyte cumulus mass, adhesion and binding to the zona pellucida, penetration through the zona pellucida, and fusion with the oocyte. Hyaluronidase, which is present in the sperm head, is known to be essential for the decomposition of the oocyte cumulus mass. Interestingly, two types of hyaluronidase—SPAM1 and —Hyal5 have been discovered in rodents, while only SPAM1 has been reported in other mammals such as pigs and humans.
The Development of an Indirect ELISA Test Using the Recombinant Protein NcSRS2 to Detect anti-Neospora Caninum Antibodies in Humans
Leandra Marla Oshiro; Rodrigo Casquero Cunha; Pâmella Oliveira Duarte; Alceu Goncalves dos Santos Junior; Cristiane Camargo Sanches; Fábio Pereira Leivas Leite; Ana Rita Coimbra Motta-Castro; Renato Andreotti*
Neosporosis is a disease caused by the Neospora caninum protozoan, an intracellular parasite belonging to the same family of Toxoplasma gondii, being confused with it until 1988, when it was described and classified. This parasite is responsible for bovine abortions and canine neuromuscular diseases. It is present in many countries and there is a variety of intermediary hosts in its life cycle, including domestic and wild animals, being transmitted vertically or horizontally.
Study on the Prevalence of Subclinical Mastitis and the related Bacterial Flora in the Raw Milk of Primiparous Indigenous Greek Goats
Tzora Athina*, Voidarou Chrissa, Giannenas Ilias, Tsinas Anastasios, Skoufos Ioannis
The objective of this study was to investigate the occurrence, prevalence and etiology of subclinical mastitis in primiparous dairy goats of indigenous Greek breeds. A total number of 340 healthy primiparous goats were used. Milk samples were taken directly from both udder cistern from individual goats and were subjected to bacteriological analysis. Bacterial growth was observed in 34.57% of total milk samples. From the latter figure 51% concerned only the half - udder that was affected. Bacterial isolates were identified on the basis of colonial morphology, Gram staining and automated phenotypic identification system Vitek 2 (bioMérieux).
Factors Associated with Puppy Morbidity and Mortality among the Breeding Kennels in Nairobi and its Environs in Kenya
Andrew Konde Matole*, George Karuoya Gitau, Japheth Chesire Kiptoon and Daniel Waweru Gakuya
Dogs are extensively employed in security and protection of property both in urban and rural areas. Besides, many other dogs are also kept as pets for companionship in urban families providing a unique, intimate, emotional bond and relationship that people share with their pets giving important meaning to their lives. Majority of puppies, however, never survive puppyhood and die within 6 months after birth. Majority of them die because of worm infestation, viral or bacterial infections, trauma, abnormalities or poor mother/puppy bond. The objective of this study was to determine the factors associated with puppy morbidity and mortality in breeding kennels in Nairobi and its environs in Kenya. The study was done for a period of 6 months.
Occurrence and Factors affecting Chronic Gastrointestinal Signs after Acute Gastric Dilatation-Volvulus Surgery in Dogs
Pia Björkenheim*, Anna Hielm-Björkman, Pirre Lamposaari, Anna-Maija Virtala, Thomas Spillmann, Outi Laitinen-Vapaavuori
To date, several studies have assessed risk factors for the development of gastric dilatation-volvulus (GDV) in dogs. However, the association between chronic gastrointestinal (GI) signs and acute GDV has rarely been investigated. The purpose of this retrospective case-control study was to investigate the occurrence of chronic GI signs in 103 dogs after acute GDV surgery performed between January 2004 and May 2008 and to identify possible risk factors associated with such signs. After GDV surgery, 54.4 % of the dogs suffered from at least one chronic GI sign.
In Vivo Comparison of the Osteogenic Properties of Equine Mesenchymal Stem Cells From Periosteum, Muscle, and Bone Marrow with and without
Demineralized Bone Matrix
Rodolfo Nino-Fong, Blanca P. Esparza Gonzalez, Enrique Aburto, Laurie A. McDuffee*
Bone healing in horses is often problematic because the high forces associated with trauma and soft tissue disruption lead to poor healing. Limitations in this process have led to research of new methods to promote bone healing in horses. We evaluated the ability of equine MSCs with scaffolds to produce new bone in nude rats after intramuscular surgical implantation of a combination of mesenchymal stem cells and scaffolds.
Impact of Milk Somatic Cell Count on Escherichia coli Growth in Vitro
Monique Lind-Böhm, Eva Kalivoda, Holm Zerbe, Wolfram Petzl*
Bovine mastitis is of major economic impact on global dairy industries. Overshooting Escherichia coli (E. coli) growth due to impaired function of polymorphonuclear cells (PMN) increases severity of acute mastitis. During mastitis, PMN account for the majority of somatic cells in milk. Besides PMN, soluble antimicrobial factors are secreted into milk. The objective of the current study was for how long and to what extent immigrating PMN and soluble factors in milk can inhibit E. coli growth in vitro.
Type I Interferons and Their Efficacy in Treating Feline Retroviral Diseases: A Review
Gomez-Lucia, Esperanza*, Collado, Victorio M, Miró, Guadalupe, Martín, Sonsoles, Domenech, Ana
Interferon is one of the molecules that the organism has ready for fighting viral infections, as it forms part of the innate immune system. In this review we briefly describe why and how the different types of type I interferon (IFN-I) prevent viral infection, some of the mechanisms that viruses have for evading these actions, and their action on cells, both healthy and tumoral. The second part of the review focuses on the effect of IFN-I on retroviral infections, specifically on their use for treating feline immunodeficiency and feline leukemia viral infections.