Immune response to immunodominant Mycobacterium tuberculosis antigen ESAT6- derived peptide is HLA-haplotype dependent
Michele Smart, Marshall Behrens, Luckey David, Catherine Conway, Veena Taneja
The antigenic proteins of Mycobacterium tuberculosis (Mtb) have been defined. We used synthetic peptides of secreted antigens, early secreted antigenic target 6 (ESAT-6) and cultural filtrate protein-10 (CFP-10), of Mtb and characterized the immune response in context of HLA genes. Humanized mice lacking endogenous class II molecules but expressing various human DR and DQ HLA transgenes singly or as a haplotype were used to study the HLA-mediated immune response to peptides.