Volume 2  Issue 2

Volume 2  Issue 2

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Research Article

Effects of IL-8 and TIMP-2 on In Vitro Term Canine Trophoblast Migration, Invasion, and Proliferation

Justine M. Gullaba, Persia R. Neumann, and Michelle A. Kutzler*

Abnormal canine trophoblast physiology in late gestation can result in postpartum complications including subinvolution of placental sites and placental retention. Understanding normal late gestation canine trophoblast physiology provides a basis for future studies aimed at characterizing postpartum placenta-related disorders. The objective of the current study was to characterize term canine trophoblast migration, invasion, and proliferation in vitro. 

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Research Article

Demecolcine Can Overcome Rat Oocyte Spontaneous Activation

Jie Zhu*, Dasari Amarnath, Inchul Choi, Yukio Tsunoda, Keith Campbell*

To inhibit oocyte spontaneous activation is an important key for the success of rat somatic cell nuclear transfer. In order to achieve this we focused on optimizing rat oocyte activation with strontium chloride (SrCl2), timing of oocyte activation and extension of in vitro culture of rat oocytes without compromising their developmental competence. First, we proved that 10mM SrCl2 combined with either 5μg/mL CytochalasinB (CB) or 2mM 6-dimethylaminopurine (DMAP) could equally, effectively activate rat oocytes and resulted in blastocyst rate of around 50%, also no difference in the mean cell number/ blastocyst was observed between the two groups.

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