Volume 3 Issue 1

Volume 3 Issue 1

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Research Article 

Structural Characteristics of rocG and ure Genes of a Novel Screened Bacillus subtilis natto Strain

Bingjun Qian, Bei Huang, Guangyan Song, Yinyun Ni, Xiaomin Yao, Glenn M. Young, Jianhua Zhang*

Natto is considered to be a health-enhancing food mainly because of nattokinase, which can lead to a mild enhancement of fibrinolytic activity in rats and dogs after oral administration. It has distinctive smell partly caused by the high amount of ammonia produced during the secondary fermentation.

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Research Article
Expression of Protein Tyrosine Kinase c-Src by Means of Protein Tyrosine Phosphatase in Pichia pastoris

Pham Ngoc Tu, Lei Shi, Xiangshan Zhou*, Yuanxing Zhang*

Active non-receptor Src protein tyrosine kinases are usually expressed with very low yield due to protein toxicity associated with its kinase activity. In this study, Src was expressed by means of Protein Tyrosine Phosphatase 1B (PTP1B) to facilitate the production of active Src by neutralizing their apparent toxicity  to cells in Pichia pastoris. Several recombinant Src-producing strains were constructed, and they displayed the catalytic activity of protein tyrosine phosphatase. The plasmid with c-Src fused with a his-tag and Green Fluorescent Protein (GFP) was transformed into P. pastoris with/without PTP1B, resulting in five recombinants with different expression levels.

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