Volume 3 Issue 1
Microarray Analysis in the Liver and Kidney of Atlantic halibut (Hippoglossus hippoglossus) following Immunostimulation with a Commercial Vaccine Against Vibrio (Listonella) anguillarum and Aeromonas salmonicida
Kyoung C. Park*, Jane A. Osborne, Laura L. Brown, Stewart C. Johnson
The response of Atlantic halibut (Hippoglossus hippoglossus) to immunostimulation with a commercial vaccine was investigated using an Atlantic halibut cDNA microarray. In this study fish were immunostimulated with the commercial injectable vaccine and held at 10-12oC. At 1, 2, 7, 14 days post-immunostimulation liver and kidney tissues were collected and mRNA was purified and used for microarray, quantitative real time-PCR (qRT-PCR) and reverse transcription-PCR (RT-PCR) analysis. Of the 381 genes on the microarray, we identified 83 different genes that were differentially expressed (≥2-fold change, p<0.05) in at least one of the samples: 44 in liver, 48 in kidney and 8 in both.
The Interaction of Tritium with Some Types of Aquatic Plants
Lydia Bondareva, Margarita Chebotina, Mikail Subbotin
Tritium is the least toxic radionuclide. The main contribution into the total tritium content into ecosystems is made by technogenic tritium which is due to the operation of nuclear fuel cycle enterprises. The tritium content in the ecosystem of the River Yenisei is connected with its background values as well as with tritium entering the water ecosystem as a result of the operation of the Mining and Chemical Combine, MCC Rosatom. Presented here are the investigations of the possible transformation of tritium interacting with certain species of aqueous plants – submerged macrophyte Elodea canadensis and aqueous plant, floating on the surface of water reservoirs, Lemna minor.
Feeding Behaviour of Larval European Sea Bass (Dicentrarchus labrax L.) in Relation to Temperature and Prey Density
Stefano Malavasi, Giacomo Cipolato, Carla Cioni, Patrizia Torricelli, Arianna Manciocco, Enrico Alleva, Mattia Toni*
The feeding behaviour of larval European sea bass (Dicentrarchus labrax, L.) was analysed in relation to temperature and prey density under controlled laboratory conditions with the aim to assess the ability of larval fish to change the feeding tactic as a response to environmental changes. Larvae were acclimated for 20 days at three different temperatures (19, 22 and 26°C), and their feeding behaviour was then video-recorded in experimental trials, at two prey densities, consisting of swarms of 400/l and 1440/lArtemia nauplii.