Volume 3 Issue 1

Volume 3 Issue 1
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Research Article

Over-Expression of Pokeweed Antiviral Protein Increases Plant Systemic Resistance Against Tobacco mosaic virus Infection in Nicotiana benthamiana

Feng Zhu*, Yang-Kai Zhou

Pokeweed antiviral protein (PAP) is members of the type I ribosome inactivating proteins (RIPs) that are isolated from the extracts of pokeweed plant (Phytolacca americana). Our previous studies suggest that exogenous application of PAP increases plant systemic resistance to Tobacco mosaic virus (TMV) infection. In this report, in order to further investigate the role of PAP in plant systemic resistance response against virus infection in Nicotiana benthamiana, N. benthamiana leaves that overexpressed PAP-II gene (35S: PAP-II) was generated through Agrobacterium-mediated transient expression.

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____________________________________________________________________________________________________________________________________                                        Research Article

Evaluation of Oxidative and Salt Stress Induced Mitogen Activated Protein Kinase Genes in Arabidopsis thaliana by Bioinformatical Approach

Seher Yolcu, Gizem Dimlioğlu, Süleyman İlhan1, Filiz Ö zdemir1, Melike Bor*

Mitogen activated protein kinases (MAPKs) are known to be involved in many stress signaling pathways, including oxidative and salt stresses. In the current study, MAPK genes of A. thaliana were selected from UniProt database, their expression levels were analyzed by AtGenExpress Microarray database and classified with DAVID Bioinformatics on-line software according to their molecular functions in response to oxidative and salt stresses. Activation of oxidative and salt stress response genes which belong to MAPK signal cascade were investigated using gene ontology (GO) terms and expression profiles.

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