Addiction Disorders and Therapy

Evaluation of abuse drugs tests variations in fresh biological samples of abusers

*Majid Rezaei Basiri
Department Of Pharmacology And Toxicology, Tabriz University Of Medical Sciences, Iran, Islamic Republic Of

*Corresponding Author:
Majid Rezaei Basiri
Department Of Pharmacology And Toxicology, Tabriz University Of Medical Sciences, Iran, Islamic Republic Of

Published on: 2019-02-12


Background and Aim: In this study N=450 fresh blood and urine samples of male abusers were employed to distinguish abuse drugs and some treatment drugs. All of the study population were male and their age range was Mean ±SD = 40±20. All study population filled satisfy form to participating in study.
Methods: Some of abuse drugs were distinguished in this study which were Heroin, Morphine, Cannabis, Methadone, Tramadol, Beprexin, Amphetamine, Metamphetamine, MethylenDioxy metamphetamine, Cocaine, Phencyclidine, Benzodiazepine, Three cyclic antidepressant, Phenobarbital… The diagnosis tests methods were determined by abusers’ dependency on treatment conditions. All data were collected and analyzed by one-way ANOVA and t-test before and after detoxification.
Results: When comparing the test results p-value 0≥0.05 was considered as significance level. Treatment duration follow-up of all blood and urine samples of the abusers indicated negative results. The results of this study showed that the blood samples of abusers are suitable to indicating positive results.
Conclusion: According to the findings of the present investigation rapid strip tests were useful in blood samples and TLC was accessible confirmation method to abusers’ urine samples. We purpose that this method will be suitable to distinguish of abuse drugs in other biological samples in future.


Abusers; Fresh Biological Samples; Rapid Strip Tests; Detoxification; Thin Layer Chromatography


Today, addiction epidemy has changed to serious social challenge and problem even in developed countries and in the poor societies social damages are consequences of increased addicted population. Statistical studies and appropriate treatment and diagnosis methods will be highly efficient in line with prevention of increasing addicted population. Scientific progress and advent of new sciences have resulted in production of new kinetic addictive substances in industrial communities. This is while during recent decades’ addiction epidemy used to involved traditional substances such as opium. Different studies have been conducted in western and weak countries in line with diagnosis, treatment and prevention of traditional and industrial addictive substances and still they are conducted currently. It seems that fast and cost-effective methods are important in addiction control and inhibition process. Based on new studies, use of fresh biologic sample of the addicted people has been proposed [1,2,3]. Hence, employment of the above mentioned methods specially in developing and highly populated countries will be cost effective. Use of advanced experimental tools is essential in additives and abuse drugs diagnosis; however, they are not economically available all the times. Hence and according to what was mentioned earlier we propose use of initial facilities such as Immuno-chromatography diagnosis strips as effective in blood and urine samples and then layer chromatography as effective in urine samples diagnosis and then we develop the research procedure and framework based on them [1,2,3,4,5].

Methods and materials

In this research study a number of 450 male addicts who had addictive substances and drugs abuse such as opium, heroin, methadone and cannabis were selected and above mentioned substances abuse form along with research participation consent form were filled for them. Each addicted subject was asked to give a fresh sample of his blood and urine to the laboratory before detoxification. Blood samples were centrifuged (4000 prm) for four minutes and then the supernatant liquid (i.e. blood serum) was isolated to do the tests. Some of abuse drugs were distinguished in this study were Heroin, Morphine, Cannabis, Methadone, Tramadol, Beprexin, Amphetamine, Metamphetamine, MethylenDioxy metamphetamine, cocaine, Phencyclidine, Benzodiazepine, Three cyclic antidepressant, Phenobarbital… The blood serums were tested by rapid strips, sensitivity of which is 300 Ng/ml to abuse drugs and then the results were recorded. In this study, some blood and all of the urine samples of addicts who were detoxified without using drug were tested after improvement using above-mentioned method and the results were recorded after treatment. Moreover, for detoxification confirmation all urine samples after treatment were tested by thin layer chromatography and their results were recorded, too. In this research, all male addicts participating in the study gave about 100cc urine sample and 100cc of fresh blood sample to the laboratory for being tested before and after treatment [1,2,3]. All of the addicts had smoking, alcohol, pain-relief and sedative medicines history. Majority of the addicts had relatively long history of using addictive substances or drugs for more than five years. Some biochemical tests such as liver enzymes experiments were conducted on the blood samples of the study addictives and then their results were recorded. Moreover, some studies were also carried out to investigate anemia level of the addicts. Results of all above-mentioned tests have been shown in the table 4 [1,2,3,4,5,6,7].


In this research all data were obtained from questioners and related tests results. Table 1 shows number of some of the drugs along with tested abuse drugs among the study addicts. As it can be seen in the table use of addictive substances such as heroin, opium and grass drugs is becoming prevalent. Table 2 shows number of positive and negative results of prevalence of addictive substances (e.g. heroin, opium and grass drugs…) based on the addicts age and use history before detoxification and after treatment among urine samples and according to the chromatography tests. Table 3 shows number of positive and negative results of prevalence of some drugs and addictive substances in the addicts’ blood samples before detoxification and after treatment among their blood samples based on rapid strip diagnosis tests. Table 4 shows confirmatory tests results and drug interventions in the urine samples of the addicts with heroin, glass and opium use after detoxification and treatment period based on doing thin layer chromatography. In this study after data collection, results were compared before and after detoxification periods and then oneway ANOVA and t-test statistical analyses were conducted on the data and finally p≤0.05 was considered as significant for all statistical population [6,8,9,10,11,12,13,14].


According to these study abusers who chronically and persistently consume abuse drugs or other treatments so these substances and medications will found in their body biological fluids, and then the searching of these drugs in their body fluids are often accessible. clinical situation and selection of drug treatment and distinguish methods are varied in different countries of the world [1,2,3,4,5,6,7,8,9,10,11,12]. According to clinical situation of abusers and facilities in different countries of middle east such as Iran, diagnostic and treatment in these regions are different. Because it might be in the abusers’ urine samples positive and false negative results can be found so Using blood samples are suitable to diagnose and confirm the presence of addictive drugs. because Nowadays we need the alternative and rapid and cheap fecible methods to distinguishing of over dose drugs are in drug poisoning in drug abuse treatment clinics and other hospitals and forensic medicine [14,15,16,17,18,19]. This study recommends that without using other complicated instruments the detection and diagnosis of other medicinal or drugs poisoning with Rapid strip test kits with above 300 ng/mL sensitivity are usefulness in biological samples such as blood samples in

                                                                                 Table 1: Shows number of some of the abuse drugs along with tested between the study population.

                                                                                Table 2: Urine rapid strip test results during the period of before and after detoxification in population study of men groups.

                                                                               Table 3: Rapid strip tests results of Blood samples during before and after detoxification

                                                                               Table 4: Urine test results have shown drug interaction after detoxification process of some cases.

hospitals and forensic medicines. Table3 clearly showed importance of rapid strip tests results in blood samples of abusers in this investigation [1,3,7,8]. However according to these study findings the use of TLC (thin layer chromatography) is very suitable to confirm of drug interactions in urine samples of abusers. Some of these studies demonstrate useful results for long-term treatment of methadone and buprexin and other medications such alcohol, opiates, methamphetamine… in abusers. In the all of clinical diagnostic laboratory centers such as addiction rehab centers undervision of welfare organization and addiction clinics and psychiatric hospitals, clinical laboratories, pharmaceutical laboratories of toxicology and forensic departments routinely use from liquid phase and solid phase extraction techniques to distinguish drugs abuse such as opioids in urine samples by the thin layer chromatography (TLC), and serum titers of some other blood parameters showed normal or dysfunction of all abusers liver and their renals’s through application of clinical laboratory tests methods in Iran [14,18,19,20,21,22].


Depending on the strategy for the treatment of abusers’ buprenorphine and methadone maintenance therapy are used. Always blood and urine tests will be positive for these substances duration of MMT course and so their abuse conditions or their detoxifications. We conclude that between all drugs progressive analytical methods the cheapest and easiest tests of opioids and other drugs in urine and blood samples is strip test for rapid diagnosis and TLC (thin-layer chromatography) is accessible method to drugs abuse detection, also doing test on blood samples have importance to immediate distinguishing of drugs abuse and other drugs poisoning in clinical laboratories. The essential function of Rapid strip test kits is Immunochromatography. They are made and accessible and cheaper than other methods in Iran [3,18]. According to background of this study, which will be recommended that to detection and diagnosis of other medicinal or drugs poisoning using of blood samples and rapid strip test kits with sensitivity above 300 ng/mL are available [11,13,14,17].


t We acknowledge that all abusers to participation of them in this investigation. We have special thanks of from all clinics that work undervision of welfare organization. We are grateful from Tabriz/Iran welfare organization to financial support of this study.


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