International Journal of Clinical and Medical Imaging

Evaluation the effectiveness of Multiplex PCR system(Septitest) for the rapid detection of blood pathogens in late onset sepsis of Extremely Low Birth Preterms

*Cekmez F
Department Of Neonatology, Acibadem Mehmet Ali Ayd?nlar University School Of Medicine, Istanbul, Turkey

*Corresponding Author:
Cekmez F
Department Of Neonatology, Acibadem Mehmet Ali Ayd?nlar University School Of Medicine, Istanbul, Turkey
Email:ferhat.cekmez@acibadem.com

Published on: 2019-04-18

Abstract

Sepsis is still a major cause of morbi=dity and mortality in the neonatal period, particularly in preterm infants. Because of difficulties in determining the infections, many biomarkers are being searched for early diagnosis. We evaluated the clinical value of a multiplex-real-time-PCR assay in episodes of suspected late-onset sepsis in ELBW preterms. A total of 30 ELBW preterm with high probable sepsis were included in the study. The qPCR in this study showed 46% positivity in patients but in positivity in blood culture is %35. The concordance of positive blood cultures to detect the bacteria in the same multiplex qPCR in patients with study group was 76%. Our studies showed that multiplex PCR tests can be a potentially useful tool in preterm sepsis.

Keywords

Preterm; Sepsis; Multiplex pcr

Introduction

Sepsis is a clinical syndrome, caused by many physiologic changes of systemic inflammation, which are due to the proven or suspected infection [1]. Sepsis is still a major cause of morbidity and mortality in the neonatal period, particularly in preterm infants. Early signs and symptoms of infection are generally occult and can easily be overlooked. However, although the onset of the disease is often unspecific, the clinical course may be fulminant, leading to septic shock, disseminated intravascular coagulation, and death within hours of initial clinical symptoms [2]. Because of difficulties in determining the infections, many biomarkers are being searched for early diagnosis, but none of them could have reached enough achievement up to now. Thus, much efforts have been focused to find effective and sensitive markers for sepsis [3].