Jacobs Journal of Anatomy and Physiology

Changes in Cytokines Status of Patients with Recurrent Pulmonary Tuberculosis Receiving Chemotherapy

*Dmytro O. Butov
Department Of Phthisiology And Pulmonology, National Institute On Psychiatry And Pulmonology , Kharkiv National Medical University, Kharkiv, Ukraine

*Corresponding Author:
Dmytro O. Butov
Department Of Phthisiology And Pulmonology, National Institute On Psychiatry And Pulmonology , Kharkiv National Medical University, Kharkiv, Ukraine
Email:dddimad@yandex.ua

Published on: 2018-05-20

Abstract

Background and objective Select cytokines in patients with recurrent TB receiving anti-TB chemotherapy were compared to the levels in healthy individuals in order to assess the balance between inflammatory and anti-inflammatory responses. Materials and methods Cytokines, IL-2, IL-4, IL-8, IL-10 and IFN-γ, were examined by quantitative ELISA (pg/L) in patients with recurrent TB (1st group; N=100) and healthy individuals (2nd group; N=30). All patients received standard TB drugs: Isoniazid (0.3 g); Rifampicin (0.6 g); Pyrazinamide (2 g); Ethambutol (1.2 g) and/or Streptomycin (1 g). Results At baseline the 1st group had serum levels of IL-2 (39.44±0.71); IL-4 (9.55±0.24); IL-8 (21.14±0.32); IL-10 (40.04±0.74) and IFN-γ (106.20±0.67); 2nd group had IL-2 (21.60±0.80); IL-4 (29.99±1.27); IL-8 (9.96±0.62); IL-10 (50.25±1.26); IFN-γ (63.82±2.27). After 2 months, there was a significant decrease in pro-inflammatory cytokine levels in the 1st (IL-2: 29.59±0.55; IL-8: 18.22±0.22; IFN-γ: 71.14±1.21). Conversely, IL-4 increased in 1st to 16.68±0.44 and IL-10 – 48.53±0.87 (p<0.05). Conclusion Prior to the study initiation patients with TB had higher IL-2, IL-8, IFN-γ and lower IL-10 and IL-4 content than healthy controls. Two-month chemotherapy produced significant reduction in pro-inflammatory cytokines and increase in anti-inflammatory IL-10 and IL-4, with levels approaching those of healthy controls. Thus, TB drugs appear to have the anti-inflammatory effect in TB patients, which was predictive of positive clinical outcome.

Keywords

Recurrent Pulmonary Tuberculosis, Cytokines, Interleukin-2, Interleukin-4, Interleukin-8, Interleukin-10, Interferon-?

Introduction

Tuberculosis (TB) occupies one of the leading positions among the most common infectious diseases. Despite the stabilization of morbidity, there is a difficult epidemiological situation with TB due to the increasing of proportion of severe advanced forms and rate growth of relapse in the total incidence of TB. In addition, the issue of pulmonary tuberculosis relapse has been remaining of great importance due to the consistently high incidence of tuberculosis reactivation in patients with active tuberculosis history for many years. The body immune system, in which lungs perform a significant role, provides systemic and local reactions to antigenic impact of different genesis. Cell interactions in the immune response are provided by cytokines system. Cytokines — are secretion products of cells involved in immune processes to identify cellular interaction. They provide pro- and anti-inflammatory, immunostimulatory, immunosuppressive, hematopoietic effects acting through receptors on the of target cells surface.