Jacobs Journal of Anatomy and Physiology

Comparison between aerobic and anaerobic training influence on s-klotho blood levels following 60 min aerobic bout

*Moran S. Saghiv
Department Of Exercise Science, University Of Mary, North Dakota, United States

*Corresponding Author:
Moran S. Saghiv
Department Of Exercise Science, University Of Mary, North Dakota, United States

Published on: 2015-04-20


The purpose of the present study was to revise and compare the association between s-Klotho serum and IGF-1 levels in elite anaerobic and aerobic trained young adult athletes, following 60 min of aerobic bout at 75% of their maximal work capacity. Methods Thirty elite athletes subjects were recruited: 15 elite anaerobically trained sprinters (24.4±1.0 years) and 15 elite matched group aerobically well trained (24.7±1.0 years). Subjects underwent maximal oxygen uptake test. Blood samples were drawn at rest and following 60 min of aerobic bout at 75% reserved heart rate, from a forearm vein after overnight fasting, s-Klotho levels in the serum were analyzed using an α-Klotho Enzyme Linked Immunosorbent Assay ELISA kit, while, IGF-1 was measured by a chemiluminescent immunometric method. Results: At rest a significant (p<0.01) differences were noted between the aerobic trained and anaerobic trained athletes for s-Klotho (672±38 and 442±24 pg•mL-1 respectively) and IGF-1 (65±7 and 94±12 mmol•L-1 respectively). Following 60 min aerobic exercise, an interaction effect (p<0.01) was obtained for s-Klotho and IGF-1 between the groups: in the aerobic group both variables were changed significantly from base level while in the anaerobic athletes s-Klotho increased significantly (p<0.05) and IGF-1 was significantly (p<0.05) reduced (566±32 pg•mL-1 and 85±8 mmol•L-1 respectively). Conclusions S-Klotho and long lasting aerobic exercise training are factors that may promote upgrading capacities of the young adults. However, being a highly anaerobically active sprinter suggests that there is no association between anaerobic vigorous exercise training and decreased risk factors for major chronic diseases.


IGF-1; sprinters; anaerobic training; aerobic training


Soluble-Klotho (s-Klotho) is a powerful longevity protein that has been linked to the prevention of muscle atrophy, osteopenia, and cardiovascular disease. S-Klotho is a transmembrane protein which can be cleaved, shed and act as a circulating hormone. Strenuous all-out exercise training increase lactic acid levels in the blood and active muscles and increase insulin-like growth factor-1 (IGF-1) levels in the blood, primarily due to a substantial major increase in plasma catecholamine concentrations. Indirect data have supported the concept that IGF-1 may be atherogenetic because it can induce vascular smooth muscle cell proliferation in vitro. But, the secreted s-Klotho protein can regulate multiple growth factor signaling pathways, including insulin/IGF-1, while, overexpression of s-Klotho increases lifespan.