Jacobs Journal of Bone Marrow and Stem Cell Research

Collagen Nanofibers Induce Spontaneous Osteogenic Differentiation of Rat Bone Marrow Stromal Cells

Published on: 2015-09-07

Abstract

Collagen nanofibers have excellent potential for use as scaffolding material for bone tissue engineering. This study evaluated nanofiber sheets formed by electrospinning of type I collagen. Rat bone marrow stromal cells (BMSCs) were cultured, expanded and seeded onto the nanofiber sheets, tissue culture plastic or collagen coated surfaces. Unseeded nanofiber sheetsserved as controls. The BMSCs were cultured for up to five weeks and their attachment, viability and differentiation into osteogenic lineage were characterized using Fluorescent Microscopy (live/dead assay), histology (Von Kossa), and Scanning Electron Microscopy (SEM). The results demonstrated that the BMSCs attached and spread well on all collagen surfaces confirming biocompatibility. After five weeks of culture without differentiation medium, BMSCs differentiated into osteoblasts and produced calcium minerals on the collagen surfaces and within the nano-scaffolds but not on the tissue culture plastic.The mineralization was confirmed by Genesis X-Ray microanalysis (EDAX). The results of this work demonstrated that extracellular protein collagen type I can serve as a single stimulus for BMSCs differentiation. Importantly, this study showed that collagen nanofibers can be used as scaffolds for osteogenic differentiation of BMSCs in vitro without the addition of differentiation stimuli.

Keywords

Collagen, Nanofibers, Mineralization, Rat Bone Marrow, Stromal cells, Osteogenic Differentiation