Evaluation of the Diagnostic Role of Human Telomerase Reverse Transcriptase (Htert) and Microrna 122a in Hepatocellular Carcinoma Patients
Published on: 2019-02-18
Background: Hepatocellular carcinoma (HCC) is currently the fifth most common solid tumor worldwide and the third leading cause of cancer related death. miRNAs are 19- to 25-nucleotide-long RNAs, able to bind complementary sequences in 3′-untranslated regions (3′-UTR) of several target mRNAs to induce their degradation or translational repression. miR- 122a, a hepato-specific miRNA, resulted down-regulated in the majority of HCCs and in all examined HCC-derived cell lines. The high frequency of aberrant regulation of these miRNAs in HCC versus non-tumor liver suggests that they might play an important role in hepatocarcinogenesis. Telomeres are specialized DNA–protein structures that cap the ends of linear chromosomes. Telomerase is a large ribonucleoprotein (RNP) complex that maintains telomeric DNA. Over 80% of human cancers show an activation of telomerase. Aim of the work: The present study was performed to evaluate the genetic role of miRNA 122a and Telomerase as reliable, non – invasive biochemical markers for early detection of hepato-cellular carcinoma Subjects and Methods: Analysis of the expression level of mature miRNA 122a (miR- 122a) and Telomerase in serum of 30 HCC patients ,30 HCV patients and 20 healthy subjects as control using quantitative reverse-transcription real time PCR (qRT-PCR). Results: Concerning miRNA-122a, the median fold change was statistically significant elevated in HCC patients when compared to both HCV and control groups. There was significant positive correlation between serum miRNA 122a and AST in HCV group. Regarding Telomerase, there was significant elevation in median fold change of serum Telomerase in HCC patients when compared to both HCV and control groups. A significant positive correlation was found between serum telomerase and total & direct bilirubin in HCC group. Conclusion: Current data suggest significant increase in miRNA 122a and Telomerase in serum of HCC patients.