Jacobs Journal of Experimental Cardiology and research
Antibacterial and Anti-Inflammatory Properties of Natural Herbal Oil Formulation (NHOF)
Published on: 2019-06-25
Previously we studied the effect of this formula against white spot syndrome virus which was published in Journal of Pharmacognosy and Natural Products (Jha et al., J Pharmacogn Nat Prod 2016 and 2:4) and that gave us the insight to investigate the other effects of this formula (NHOF). Natural Herbal Oil Formulation (NHOF) produced in the form of secondary metabolites from plants is originally used in traditional medicine. In the present work, the anti- inflammatory, -microbial and -fungal potential of-13 formulation contained Lavandula latifolia, Pinus sylvestris, Jasminum officinale, Citrus limon, Prunus avium, Viola odorata, Gardenia jasminoides, Cocos nucifera, Rosa damascene and Eucalyptus globulus was evaluated. For this, PBMCs from healthy donors were treated with different concentrations of Natural Herbal Formulation and cytokine content (IL-1β, IL-6, IL-10, TNFα and IFNγ) was determined in culture supernatants. The antimicrobial activity of natural formulation was studied by disc diffusion and broth dilution methods with using six bacterial (Staphylococcus aureus ATCC-6538, Staphylococcus epidermidis ATCC-12228, Escherichia coli ATCC-8739, Salmonella enteric serovar Typhimurium ATCC-14028, Pseudomonas aeruginosa ATCC-9027, Micrococcus luteus ATCC-10240) and two fungal (Candida albicans ATCC-10231, Candida albicans NCTC-885-653) strains. We observed a prominent suppression of proinflammatory cytokines production by LPS-primed PBMCs in the presence of Natural Herbal Formulation. A Natural Herbal Oil Formulation mixture showed moderate antimicrobial effect. Therefore, our results suggest that Natural Herbal Formulation may serve as a promising pharmaceutical agent with combined anti-inflammatory, -microbial and –fungal action.