Previously we studied the effect of this formula against white spot syndrome virus which was published in Journal of Pharmacognosy and Natural Products (Jha et al., J Pharmacogn Nat Prod 2016 and 2:4) and that gave us the insight to investigate the other effects of this formula (NHOF). Natural Herbal Oil Formulation (NHOF) produced in the form of secondary metabolites from plants is originally used in traditional medicine. In the present work, the anti- inflammatory, -microbial and -fungal potential of-13 formulation contained Lavandula latifolia, Pinus sylvestris, Jasminum officinale, Citrus limon, Prunus avium, Viola odorata, Gardenia jasminoides, Cocos nucifera, Rosa damascene and Eucalyptus globulus was evaluated. For this, PBMCs from healthy donors were treated with different concentrations of Natural Herbal Formulation and cytokine content (IL-1β, IL-6, IL-10, TNFα and IFNγ) was determined in culture supernatants. The antimicrobial activity of natural formulation was studied by disc diffusion and broth dilution methods with using six bacterial (Staphylococcus aureus ATCC-6538, Staphylococcus epidermidis ATCC-12228, Escherichia coli ATCC-8739, Salmonella enteric serovar Typhimurium ATCC-14028, Pseudomonas aeruginosa ATCC-9027, Micrococcus luteus ATCC-10240) and two fungal (Candida albicans ATCC-10231, Candida albicans NCTC-885-653) strains. We observed a prominent suppression of proinflammatory cytokines production by LPS-primed PBMCs in the presence of Natural Herbal Formulation. A Natural Herbal Oil Formulation mixture showed moderate antimicrobial effect. Therefore, our results suggest that Natural Herbal Formulation may serve as a promising pharmaceutical agent with combined anti-inflammatory, -microbial and –fungal action.
Throughout our evolution, plants were used as a source of products for the treatment of different illnesses. In recent years, there has been renewed interest in usage of natural compounds due to reduced efficacy of synthetic drugs and the number of adverse effects Special attention was focused on numerous plant-derived products. [1, 2] Among the plant-derived extracts, Natural Herbal Oil Formulation (NHOF) from aromatic medical plants has been attracted substantial research interest. Natural Herbal Oil Formulation is mixture of volatile compounds (mainly mono- and sesquiterpenoids, benzenoids, amino acidderivatives, phenypropanoids, etc.) with wide range of pharmacological actions, e.g. antimicrobial, antifungal, anti-inflammatory, antioxidant, hyperemic, anti-nociceptive, spasmolytic, diuretic, choleretic, and carminative effects [3, 4]. For centuries, natural products have been widely used for combating infection in various cultures. Accordingly World Health Organization, a large proportion of the human population and livestock still rely on herbal medicinal products . Nowadays, the use of natural antimicrobial agents is noticeably growing for wide variety of purposes including human and animal medication, food industry and environment protection. In our study, the Natural Herbal Oil Formulation obtained from Lavandula latifolia, Pinus sylvestris, Jasminum officinale, Citrus Limon, Prunus avium, Viola odorata, Gardenia jasminoides, Cocos nucifera, Rosa damascene and Eucalyptus globulus were selected to develop a drug with antimicrobial and immune modulatory properties. Despite the biological activities of essential volatile oil blend (Natural Herbal Formulation) compounds were separately evaluated, combination of Natural Herbal Oil Formulation constituents may represent a valid strategy to achieve better antimicrobial and anti-inflammatory effects. Essential volatile oils were traditionally used to treat many pathological conditions and become popular nowadays. Despite recent studies have reported the anti- inflammatory effect of NHOF, there is a substantial lack in understanding of how single and mixed violate oils balance the pro- and anti-inflammatory responses in culture of human cells