Association between C677T MTHFR Polymorphism and H pylori Infection among Jordanian Gastric Cancer Patients
Published on: 2018-05-09
Introduction: Methyl tetrahydrofolate reductase (MTHFR) polymorphism and H pylori infection increase gastric cancer (GC) development. So the aim of this study to evaluate C677T MTHFR gene polymorphism and investigate the possible interaction between this polymorphism and H pylori infection as a possible risk factors for GC. Material and methods: This study enrolled one hundred and twenty GC patients from Ibn-Nafees hospital Irbid (2010-2015) and one hundred and thirty eight age matched control with no family history of any cancer. DNA was extracted using DNA extraction and purification kit (Promega, USA). MTHFR C677T was detected by FV-PTH-MTHFR Strip Assay (ViennaLab, Austria). H pylori IgG was detected by ELISA (Novalisa, Germany). Results: This case control study showed that MTHFR C677T genotypes frequencies among Jordanians GC is: CC, 61.7%, CT, 34.2%; and TT, 4.2% among GC cases and 54.4%, 37.7% and 7.9% among controls; respectively. The data of this study also showed that the frequency of intestinal GC (42/120=35%) were more common than diffuse GC (26/120=21.7%) among Jordanian GC patients. MTHFR C677T polymorphism increased the risk of GC by 35% (OR =1.35, 95% CI = 0.82 – 2.22), and increase intestinal GC risk by 51% and diffused GC by 34%. MTHFR 677CT and MTHFR 677 TT genotypes interacted with H pylori infection and significantly associated with increased risk of GC (p=0.009 and p=0.009; respectively) Conclusion: MTHFR C677T polymorphism increased the risk of GC by 35%. Both MTHFR 677CT and MTHFR 677 TT genotypes interacted with H pylori infection are significantly increased the risk of GC among Jordanian.