Jacobs Journal of Genetics

Detection of Virulence-Associated Genes of Avian Pathogenic Escherichia Coli (APEC) Isolated from Broilers

Published on: 2017-12-13

Abstract

Escherichia coli is responsible for significant losses in the poultry industry. This study aimed to determine the prevalence, serotypes, the virulence-associated genes and the antimicrobials susceptibility of avian pathogenic E. coli (APEC) strains. A total of 1200 samples were collected from 200 birds (60 recently dead, 80 diseased and 60 apparent healthy broilers). Standard disc diffusion method used for determination of antimicrobials susceptibility. PCR used for the detection of virulence genes. Bacteriological examination revealed that E. coli was recovered from 842 samples with overall prevalence of 70.16%. Incidence of E. coli from fresh heart blood samples was 75%, from liver 83%, from kidney 64%, from spleen 57%, from small intestine74.5% and from bone marrow was 67.5 %. E. coli isolates belonged to Serotypes O111, O44, O55, O142, O128, O158, O157, O29 and O115. The antimicrobials susceptibility profile of the isolates showed resistance to Ampicillin, Neomycin, Doxycycline and Oxytetracycline, while ciprofloxacin and Erythromycin were effective against the isolates. PCR assay was carried out to detect the presence of phoA, iss and iutA gene, all serovars had the three genes except (O29) not possess iss gene. High prevalence of multidrug resistant avian pathogenic Escherichia coli (APEC) among broilers.

Keywords

APEC; Broilers; Egypt