Jacobs Journal of Genetics

Identifying Genetic Risk Factors for Low Ovarian Response

Published on: 2018-12-21

Abstract

Background: One of the most frustrating problems in the treatment of infertility is that of poor ovarian response to stimulation. It would therefore be advantageous to have a genetic marker that could predict low ovarian reserve at a young age before the impact of low ovarian reserve affects a woman’s fertility. Since both BMP15 and GDF9 gene mutations have been reported in women with premature ovarian failure , the purpose of the present study is to investigate the correlation between low ovarian reserve BMP15 c. A704G( p. Y235C) , BMP15 c.T443C (p.L148P ), GDF9 c.C557A (p. S186Y) ,and GDF9 c. A199C (K67E) polymorphisms .
Methods: Buccal swabs were obtained from 90 women, of whom 50 had a diagnosis of low ovarian reserve and 40 were fertile controls. DNA was extracted from the buccal swabs followed by polymerase chain reaction-sequence based tying (PCR-SBT) amplification to examine the frequencies of BMP15 Y235C , BMP15 L148P , GDF9 S186Y,and missense c.199A>C polymorphisms.
Results: No significant differences were observed when the frequencies between BMP15 Y235C , BMP15 L148P , GDF 9 S186Y,and GDF9 K67E polymorphisms among women with a diagnosis of low ovarian reserve were compared with those of control women.
Conclusion: BMP15 Y235C , BMP15 L148P , GDF9 S186Y,and GDF9 K67E polymorphisms cannot be used as markers of low ovarian reserve.

Keywords

BMP15 Y235C; BMP15 L148P; GDF9 S186Y; and GDF9 K67E polymorphisms; low ovarian reserve