Jacobs Journal of Genetics

Association between C677T MTHFR Polymorphism and H pylori Infection among Jordanian Gastric Cancer Patients

*Manar Fayiz Atoum
Department Of Medical Laboratory Sciences, Jordan

*Corresponding Author:
Manar Fayiz Atoum
Department Of Medical Laboratory Sciences, Jordan
Email:manar@hu.edu.jo

Published on: 2018-05-09

Abstract

Introduction: Methyl tetrahydrofolate reductase (MTHFR) polymorphism and H pylori infection increase gastric cancer (GC) development. So the aim of this study to evaluate C677T MTHFR gene polymorphism and investigate the possible interaction between this polymorphism and H pylori infection as a possible risk factors for GC.
Material and methods: This study enrolled one hundred and twenty GC patients from Ibn-Nafees hospital Irbid (2010-2015) and one hundred and thirty eight age matched control with no family history of any cancer. DNA was extracted using DNA extraction and purification kit (Promega, USA). MTHFR C677T was detected by FV-PTH-MTHFR Strip Assay (ViennaLab, Austria). H pylori IgG was detected by ELISA (Novalisa, Germany).
Results: This case control study showed that MTHFR C677T genotypes frequencies among Jordanians GC is: CC, 61.7%, CT, 34.2%; and TT, 4.2% among GC cases and 54.4%, 37.7% and 7.9% among controls; respectively. The data of this study also showed that the frequency of intestinal GC (42/120=35%) were more common than diffuse GC (26/120=21.7%) among Jordanian GC patients. MTHFR C677T polymorphism increased the risk of GC by 35% (OR =1.35, 95% CI = 0.82 – 2.22), and increase intestinal GC risk by 51% and diffused GC by 34%. MTHFR 677CT and MTHFR 677 TT genotypes interacted with H pylori infection and significantly associated with increased risk of GC (p=0.009 and p=0.009; respectively)
Conclusion: MTHFR C677T polymorphism increased the risk of GC by 35%. Both MTHFR 677CT and MTHFR 677 TT genotypes interacted with H pylori infection are significantly increased the risk of GC among Jordanian.

Keywords

C677T; MTHFR; H pylori; Gastric Cancer

Introduction

Methyl tetrahydrofolate reductase (MTHFR) is a key enzyme for folate metabolism. MTHFR gene encoded 5-10-methylentetrahydrofolate reductase enzyme that catalyze the reduction of 5-10 methylentetrahydrofolate to 5-methylentetrahydrofolate which is a co-substrate for the re-methylation of homocysteine into methionine [1], and finally to S-adenosyl-L-methionine. Reduced MTHFR gene activity result in low S-adenosyl-L-methionine activity that increased cancer risk [2]. Understanding MTHFR gene polymorphism may enhance understanding the pathogenesis of cancer. MTHFR polymorphism are associated with colorectal cancer [3], esophageal squamous cell carcinoma [4], chronic myeloid leukemia [5], breast cancer [6] and thyroid carcinoma [7]. Two functional polymorphisms were recorded within MTHFR gene; C677T and A1298C [8]. C677T result in a substitution of alanine for valine at amino acid 222 in exon 4 and low enzyme activity. The epidemiology of these polymorphism varies dependent on the geography and ethnicity [9].