Jacobs Journal of Genetics

Efficacy of Trichoderma Harzianum Formulations against Seedling Disease of Barley Caused By Sclerotium Rolfsii

*Md. Iqbal Faruk
Plant Pathology Division, Bangladesh Agricultural Research Institute Joydebpur, India

*Corresponding Author:
Md. Iqbal Faruk
Plant Pathology Division, Bangladesh Agricultural Research Institute Joydebpur, India
Email:mifaruk2012@yahoo.com

Published on: 2019-05-03

Abstract

The efficacy of formulated Trichoderma harzianum based Tricho-compost, seed treatment with Trichoderma spore suspension Trichoderma inocula and chemical fungicide Provax 200 WP were tested against seedling disease of barley caused by soil borne pathogen Sclerotium rolfsii in the research field of Plant Pathology Division, Bangladesh Agricultural Research Institute (BARI), Gazipur, Bangladesh during 2013-14, 2014-15 and 2015-16. Tricho-composts were prepared with mixed substrates of cow dung, rice bran and poultry refuse colonized by T. harzianum. The results exhibited that seedling mortality of barley was significantly reduced by the Tricho-compost, Trichoderma inocula and Provax 200 WP in all the years. The yield of barley was sharply increased over control due to the T. harzianum formulations and Provax 200 WP. Among the treatments, application of Tricho-compost was found more efficient in the reduction of seedling mortality and acceleration of plant growth with increased yield of barley under S. rolfsii inoculated field experiments in Bangladesh. Seed treatment with Provax 200 WP and Trichoderma inocula also showed promising in reduction of seedling mortality and increasing plant growth and yield of barley.

Keywords

Barley; Provax 200 WP; Sclerotium roflsii; Seedling disease; Tricho-compost; Trichoderma inocula

Introduction

Barley (Hordeum vulgare L.) is a cereal crop of international importance and stands fourth major food grain crop after wheat, rice, and maize in the world in both quantity produced (136 million tons) and area of cultivation (566,000 km2) [1,2]. It is a major source of food for large number of people living in the cooler semi-arid areas of the world. The crop is being used as food, animal feed and industrial raw material in food and beverages industry. However, the crop suffers significant yield losses from a biotic and biotic stresses in the world [3]. The crop can be grown in drought prone areas as well as saline areas of Bangladesh. The yield per unit area of barley at present is low in Bangladesh compared to other country. There are many biotic and a biotic factors responsible for low yield of barley in Bangladesh. Among the factors, diseases play an important role. At least 17 important diseases of the crop have been recorded in the country [4, 5] where seedling disease caused by Sclerotium rolfsii is one of the major one. Sclerotium rolfsii, an omnivorous, soil-borne fungal pathogen, causes disease on a wide range of agricultural and horticultural crops and difficult to control through conventional method such as cultural, physical, chemical etc. Chemical control of this fungus using fungicides required large amount of chemical which causes health hazardous to grower and pollution of environment and soil. Thus, biological control using antagonistic microbes alone, or as a supplement of the minimizing of chemical pesticides in the integrated plant disease management system, has become more important and alternative methods for management of disease of many crops especially disease caused by soil borne pathogens.