Jacobs Journal of Gynecology and Obstetrics

Toxicity of A Pesticide Mixture to Pregnant Mice and Their Pups with Special Concern to Ameliorative Effect of Vitamin E

Published on: 2019-03-21


This study evaluates toxicity of a mixture of three pesticides (atrazine, chlorpyrifos and endosulfan; ACE) added to the rodent diet allowing the mice to ingest the equivalent of the Acceptable Daily Intake (ADI: 0.005, 0.01 and 0.006 mg/kg body weight /day, respectively), in addition to oral administration of vitamin E (α-tocopherol; 100 ul / mouse). During gestation (21d), the mouse dams were received one of the following treatments: (a) diet free of ACE; (b) diet enriched with ACE; (c) diet free of ACE + oral vitamin E; and (d) diet enriched with ACE + oral vitamin E. During lactation, the dams were not subjected to any chemical treatments. After weaning (42d), selected organs and blood samples were collected for analyses. Compared with the control results either in dams or their pups, the ACE mixture induced high elevation in AST, ALT, ALP, urea and MDA and high decline in BuChE, SOD and CAT. The pups were more affected than the dams with respect to alterations in MDA and BuChE activities, while the opposite was achieved with respect to SOD activities. The ameliorative effect of vitamin E, based on estimating the “Amelioration Index; AI” revealed the powerful effect of this vitamin in alleviating the oxidative stress exerted by the tested mixture. The findings may encourage further work on the toxicity of pesticide mixtures, especially on pregnant and lactating animals and the reproductive effects on new born child. Also, the search for powerful antioxidants has to receive special attention.


Pesticide mixture; Oxidative stress; Vitamin E; Amelioration; Pregnant mice