Comparison study of Biodegradation of Phenanthrene by Thalassospira frigidphilosprofundus and Pseudomonas putida
Published on: 2019-01-21
The Polycyclic aromatic hydrocarbons (PAHs) are toxic and carcinogenic, it creates so many serious problems to the environment, and it shows necessity for degrading these PAHs. Using microbial transformation, it is possible to decrease the concentration of these PAHs. The aim of the present study is to analyze the biodegradation of Polycyclic Aromatic Hydrocarbons (PAHs) compounds by the bacterial strains Pseudomonas putida and Thalassospira frigidphilosprofundus, examine and find the strain having high potential for maximum degradation of PAHs. In this work, one PAHs namely Phenanthrene were studied as these show highest concentration at contaminated sites. These bacterial strains were tested under parameters of pH(6-8), inoculum size (1-3ml), temperature(200 C-400 C), and incubation period (1-11days) and the optimum growth conditions obtained for Pseudomonas putida were pH 7,inoculum size 1ml, 300 C, 7days and for Thalassospira frigidphilosprofundus were pH 7, inoculum size 1ml, 200 C, 7days respectively. By varying the concentrations of PAH compounds from 100mg/l - 400mg/l, degradation results of PAHs by Pseudomonas putida were Phenanthrene (35%,38%,33% and 0.7%), Thus the degradation phenomenon of PAHs gave Thalassospira frigidphilosprofunds has better degradation potential than Pseudomonas putida.
Thalassospira frigidphilosprofundus,Pseudomonas putida,Biodegradation of Phenanthrene