Pc 4 Conjugated Iron Oxide Nanoparticles as Photodynamic Therapeutic Carriers
Published on: 2018-12-22
Magnetite nanoparticles have been chosen in the CosmoPHOS-nano project as one of three types of phthalocyanine conjugated carrier particles because of their magnetic properties and biocompatibility. The main challenge was the dye-conjugation of the nanoparticles. This article does not focus on the magnetic properties but rather on the possibilities of using these conjugates in the Life- Science. Monodisperse magnetite nanoparticles, 15 nm in diameter, were prepared by thermal decomposition of iron(III) oleate in high boiling octadecene in presence of oleic acid as a stabilizer. Ligand exchange of oleic acid by aspartic acid enables water solubility. Next, amino groups were present at the outer nanoparticle’s surface which allows for further attachment of the phthalocyanine Pc 4 by standard conjugation techniques. Pc 4 producing singlet oxygen through illumination at 670 nm in water was investigated as a photosensitizer. Further photodynamic cell-killing experiments with these dye-conjugated carriers on mouse macrophage cell lines showed high efficacy with IC50-values down to 0.03 ng/µl Pc 4, i.e. 1.9 ng/µl Fe3 O4 .
Photodynamic Therapy; Magnetite Nanoparticles; Pc 4; Cell-Killing