Kinetin and N-6-Benzyladenine Influence on Cell Migration and Collagen Biosynthesis in Human Skin Fibroblasts
Published on: 2019-03-24
N-6-benzyladenine and kinetin belong to the group of plant hormones called cytokinins. Our previous work showed their positive influence on oxidative stress parameters tested in fibroblasts, therefore we decided to examine their effect on cell migration and collagen content, which wasn’t under the investigation yet. Phytohormones were added to the cultured cells for a final concentration in the range of 10-5 to 10-6 M for kinetin and 10-6 to 10-7M for N6-benzyladenine. The control cells were incubated without the test compound. Fibroblasts migration was assayed by using Wound Healing Assay. The concentration of proteins was determined spectrophotometrically as per Lowry et al (1951). Collagen content in cells and medium were determined spectrophotometrically by observation that Sirius red in saturated picric acid selectively binds to fibrillar collagens (types I to V). The results show stimulatory effect of tested compounds on cells migration and collagen biosynthesis, as well as total protein content. Kinetin and N-6-benzyladnine effectiveness demonstrated in this study in relation to the skin cells may indicate their potential therapeutic relevance, especially regarding skin diseases.