Kinetin and N-6-Benzyladenine Influence on Cell Migration and Collagen Biosynthesis in Human Skin Fibroblasts
*Agata Jablonsk Department Of Sanitary Biology And Biotechnology, Poland
*Corresponding Author: Agata Jablonsk
Department Of Sanitary Biology And Biotechnology, Poland Email:email@example.com
Published on: 2019-03-24
N-6-benzyladenine and kinetin belong to the group of plant hormones called cytokinins. Our previous work showed their positive influence on oxidative stress parameters tested in fibroblasts, therefore we decided to examine their effect on cell migration and collagen content, which wasn’t under the investigation yet. Phytohormones were added to the cultured cells for a final concentration in the range of 10-5 to 10-6 M for kinetin and 10-6 to 10-7M for N6-benzyladenine. The control cells were incubated without the test compound. Fibroblasts migration was assayed by using Wound Healing Assay. The concentration of proteins was determined spectrophotometrically as per Lowry et al (1951). Collagen content in cells and medium were determined spectrophotometrically by observation that Sirius red in saturated picric acid selectively binds to fibrillar collagens (types I to V). The results show stimulatory effect of tested compounds on cells migration and collagen biosynthesis, as well as total protein content. Kinetin and N-6-benzyladnine effectiveness demonstrated in this study in relation to the skin cells may indicate their potential therapeutic relevance, especially regarding skin diseases.
Cytokinins are one of the major groups of plant hormones. Although their effect on the biochemical changes that occur in plant cells and their molecular mechanism of action were fairly well established, there is a significant lack of information considering their effect on the growth and metabolism of animal and human cells . In our previous research we showed a positive influence of selected cytokinins – kinetin (K) and N-6-benzyladenine (BA) on oxidative stress parameters . Our recent findings also revealed that traumatic acid – a cytokinin, that in terms of chemical structure belongs to the group of fatty acids, enhances collagen biosynthesis and reduces oxidative stress in cultured human skin fibroblasts . Therefore we decided to investigate the effect of K and BA in previously established concentrations on cell migration and collagen, as a basic protein that builds the skin, content. A convenient test model for determining the influence of biologically active compounds on fundamental biochemical parameters and morphological changes in the dermis is in vitro human skin fibroblasts culture. Since the cytokinins were not studied in detail as a potential compounds that act therapeutically on human skin, the aim of the present study was to evaluate their effect on the proliferative capacity of the main cells in the dermis – fibroblasts, on the morphological changes taking place within them, on cells migration and on a number of biochemical parameters such as total protein and collagen content. Collagen is a primary protein constituent of the skin . Many active substances used in therapeutic formulations and supplements have favorable effects on metabolic processes of skin cells. Examples of such compounds are phytohormones, such as K and BA.