Journal of Molecular Biomarkers and Clinical Trials

Hyaluronic Acid and Silver Sulfadiazine Combination: Evaluation in a Second Degree Burns Rat Model

Published on: 2017-05-12


Objective: Assessing the benefit of a hyaluronic acid-silver sulfadiazine combination versus a silver sulfadiazine formulation in a rat model. Methods: A deep second degree burn was induced on the lower and upper back of 59 rats using a standardized severe burning procedure. The burn was daily dressed and responses to both treatments were assessed until complete wound closure. Macroscopic (inflammation, moisture environment, epithelization and retraction) and microscopic (dermis formation, blood vessels, collagen reorganization, early annexes) parameters were assessed.

Results: Hyaluronic acid silver sulfadiazine combination decreased and almost prevented apparition and persistence of exudate (13.6% ± 4.5 vs 81.4% ± 5.1 of treated wounds; 1.63 ± 0.26 vs 7.19 ± 0.63 days) and maceration events (18.6% ± 5.1 vs 98.3%± 1.7 of treated wounds; 2.36 ± 0.64 vs 12.76 ± 0.74 days). While no significant difference was found in terms of complete epithelization, the later appeared faster using hyaluronic acid silver sulfadiazine combination (25% of wound epithelization wasreached at 19.58 ± 0.48 vs 22.40 ± 0.65 days and 50 % of wound epithelization at 30.91 ± 0.94 vs 35.44 ± 1.41 days). Woundretraction was also achieved significantly faster when using hyaluronic acid silver sulfadiazine combination (25% was reached at 13.89 ± 1.19 vs 29.72 ± 1.28 days and 50% at 35.48 ± 1.08 vs 42.09 ± 0.92 days).

Conclusions: The hyaluronic acid silver sulfadiazine combination appears well indicated for the treatment of a deep second degree burn, probably by creating an optimal and favourable environment for an efficient wound healing process.


Second Degree Burn; Hyaluronic Acid; Silver Sulfadiazine