Open Access Journal of Genome Biology and Bioinformatics

Systems Biology of the Proteomic Analysis of Cytotoxic Gold Compounds in A2780 Ovarian Cancer Cell Line: A Network Analysis

*Alessandra Modesti
Department Of Experimental And Clinical Biomedical Sciences, University Of Florence, University Of Florence, University Of Florence, Italy, Italy

*Corresponding Author:
Alessandra Modesti
Department Of Experimental And Clinical Biomedical Sciences, University Of Florence, University Of Florence, University Of Florence, Italy, Italy
Email:alessandra.modesti@unifi.it

Published on: 2018-08-03

Abstract

Gold and ruthenium compounds hold today great promise as new metallodrugs for treatment of human ovarian carcinoma; yet, their mode of action is still largely unknown. To shed light on their molecular mechanisms in previous studies we performed proteomic analysis in the A2780 human ovarian cancer cell line, after treatment with four different gold and two ruthenium compounds. To gain a better interpretation of their cellular effects we reported here the bioinformatic analysis of proteomic data that confirmed the action of these metallodrugs on previously identified signaling pathways such as glucose metabolism, protein folding, and cell division cycle and apoptosis. In addition, this new study pointed out the involvement of novel pathways such as synthesis and degradation of purine/pyrimidine nucleotides as well mitochondrial energy metabolism. These findings strengthen the idea of energy metabolism and mitochondrial function as possible target of both gold and ruthenium compound in A2780 cell line.

Keywords

Network Analysis; Proteomics; Metallodrugs; Systems Biology; Ovarian Cancer Cells

Introduction

The protein composition represents the functional status of biological processes at a given time. Proteomics methods may provide an analysis of the alteration induced by drugs on protein expression and these alterations may be related to the mode of action of the drugs themselves. Consequently, identification of protein alterations specific to diseases by proteomic approaches has been helpful to clarify the therapeutic benefit of drugs. Moreover, proteomic profiling offers the opportunity to identify proteins that mediate apoptotic pathways, in cells treated with cytotoxic agents.