Volume 1  Issue 3

Volume 1  Issue 3

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Research Article

Epidemiology of Swine Hepatitis E Virus in Guangxi, China and Construction of Eukaryotic Expression Plasmid with ORF2 Gene for possible Vaccine

Q Su1#, B Li#, W Zhao*, Y. Qin, J Liang, B Lu, A Xiao, Q Duan, Y He, D Jiang, W Huang

Hepatitis E (HE) is a zoonotic disease that can be transmitted through food or water contaminated from swine with HE virus (HEV) as an important natural reservoir host. This paper presents emperical data of epidemiological testing conducted at 14 cities in Guangxi, China, where random samples of 1387 serum samples were collected from the swine population. ELISA test for total antibodies of HEV showed that 1107 were positive in swine serum samples, with an average positive rate of 79.81%. In order to develop swine hepatitis E DNA vaccine, specific primers of immunogenic specific ORF2 gene targets for LB1, LB2, LB3 were designed and amplified using PCR assays. DNA amplicons were then directly cloned into T-A plasmid and expressed using pEASY-M1 eukaryotic expression vector system.

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Review Article

Animal Welfare Considerations: Should Context Matter?

F. Ohl* and R.J. Putman

Much consideration of animal welfare issues, as well as the bulk of welfare legislation, is closely related to context, with clear distinction between defined responsibilities towards farm-, laboratory-, companion- and wild animals. It has however been recognised that such distinction leads to inconsistencies, in that it might lead to different welfare management practices for the same rat (for example) as a pet, laboratory animal or pest.

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