Volume 1 Issue 4
Effect of Gelatin Hydrogel Sponge Loaded with Bone Marrow Mesenchymal Stem Cells and Hepatocyte Growth Factor on Peripheral Nerve Regeneration
N. Sasaki*, J.P. Seo, H. Uchiyama, S. Haneda, K. Yamada, H. Furuoka, M. Itho, M. Yanagawa, Y. Tabata
Hepatocyte growth factor (HGF) can promote nerve cell proliferation. Gelatin hydrogel sponge can act as a scaffold for drug controlled release. . This study aimed to evaluate the beneficial effects of a gelatin hydrogel scaffold (sponge) loaded with BM-MSCs and HGF on radial nerve regeneration in a defect model. Twelve healthy calves (weighing 48.7 ± 5.54kg, mean ± S.D.) were used in this study. Stem cells were derived from bone marrow of ilium and cultured into culture dishes for two weeks. 1cm of the left radial nerve was defected under inhalation anesthesia.
Cyclical Shedding and Sustained anti-Phase 1 Antibody Responses in a Goat Herd Naturally Infected with Coxiella burnetii
Alicia D. Anderson*, Tahnee J. Szymanski, Kelly A. Fitzpatrick, Joshua S. Self, Rachael A. Priestley, William L. Nicholson, Gilbert J. Kersh
Coxiella burnetii is a worldwide zoonotic pathogen that can infect a variety of species and can cause Q fever in humans. Although an infected goat herd is a risk to human health, the duration of shedding and antibody responses in infected goats have not been clearly defined. In 2011, an outbreak of human Q fever in Washington and Montana was associated with infected goats, and one herd offered an opportunity for longitudinal sampling to investigate antibody responses and shedding of C. burnetii.
Puppy Morbidity and Mortality among Breeding Kennels in Nairobi, Kenya
Andrew Matole Konde*
The dog to human population ratio is estimated to range from 1:4 – 1:11. The human population of Kenya was estimated to be slightly over 43 million in 2012. Using the dog human population ratio range of 1:8 – 1:11, Kenya today has an estimated dog population of between 3.6 and 4.8 million. Dog breeders in Kenya are in the business to produce show, companion or guard dogs with an observed stillbirth rate of about 2.3 %, pre-weaning mortality of 11.4 % and a pre-weaning culling rate of 0.9 % for German shepherd dog.
Is Sperm Hyaluronidase Indispensable in Mammalian Fertilization?
Fertilization involves several precisely coordinated steps, including sperm dispersal of the oocyte cumulus mass, adhesion and binding to the zona pellucida, penetration through the zona pellucida, and fusion with the oocyte. Hyaluronidase, which is present in the sperm head, is known to be essential for the decomposition of the oocyte cumulus mass. Interestingly, two types of hyaluronidase—SPAM1 and —Hyal5 have been discovered in rodents, while only SPAM1 has been reported in other mammals such as pigs and humans.
The Development of an Indirect ELISA Test Using the Recombinant Protein NcSRS2 to Detect anti-Neospora Caninum Antibodies in Humans
Leandra Marla Oshiro, Rodrigo Casquero Cunha, Pâmella Oliveira Duarte, Alceu Goncalves dos Santos Junior, Cristiane Camargo Sanches, Fábio Pereira Leivas Leite, Ana Rita Coimbra Motta-Castro, Renato Andreotti*
Neosporosis is a disease caused by the Neospora caninum protozoan, an intracellular parasite belonging to the same family of Toxoplasma gondii, being confused with it until 1988, when it was described and classified. This parasite is responsible for bovine abortions and canine neuromuscular diseases. It is present in many countries and there is a variety of intermediary hosts in its life cycle, including domestic and wild animals, being transmitted vertically or horizontally.