Volume 1 Issue 1
Mechanism of Ventricular Volume Improvement after Autologous Stem Cell Implantation in Acute Myocardial Infarction
Alvaro Merino* MD PhD, Antoni Gaya MD PhD, Cristina Imízcoz RN, Javier Calvo MD PhD, Teresa Jimenez-Marco MD PhD, Enrique Girona-Llobera MD PhD, Ricard Jordá, MD
We tried to obtain a significant number of CD133+ and CD34+ haematologic precursors from systemic circulation by aphaeresis, and analyzed necrosis area reduction by Tc-91 myocardial perfusion scanning and left ventricular diameters by cardiac ultrasound in a group of patients who received stem cell therapy in the first week after a myocardial infarction.
Directed Differentiation of Late Stage islet Lineages Remains a Knowledge Gap in Pancreatic Endocrine Development
Fang-Xu Jiang*, Grant Morahan
Directed differentiation of human embryonic stem cells(ESCs) and induced pluripotent stem cells (iPSCs) into approximately210 types of functional cells in our body is theultimate goal of regenerative medicine. The latter is a type of new medicine which is to replace/restore those cells lostthrough injury or disease with functional new cells. Type 1and type 2 diabetes mellitus are pandemic metabolic disorders that currently affect approximately 400 million peopleworldwide, characterized by absolute and relative loss of insulin-secreting β cells and hence are candidate diseases for regenerative medicine.
Collagen Nanofibers Induce Spontaneous Osteogenic Differentiation of Rat Bone Marrow Stromal Cells
Therese Bou-Akl*, Rebecca Miller , Pamela VandeVord
Collagen nanofibers have excellent potential for use as scaffolding material for bone tissue engineering. This study evaluated nanofiber sheets formed by electrospinning of type I collagen. Rat bone marrow stromal cells (BMSCs) were cultured, expanded and seeded onto the nanofiber sheets, tissue culture plastic or collagen coated surfaces. Unseeded nanofiber sheets served as controls.
Predictive Factors for the Outcome of Salvage Autologous Stem Cell Transplant in patient with Relapsed Multiple Myeloma
Mohamed Emara*, Ankur Sharma, Sabuj Sarker, Riaz Alvi, Waleed Sabry, Julie Stakiw, Hadi A. Goubran, Mohamed Elemary
Multiple myeloma is one of the most common hematologic malignancies in adults. In the United State, it is estimated that 24,050 patients will be newly diagnosed and 11,090 patients will die of multiple myeloma in 2014. Autologous stem-cell transplant is considered the standard of care for patients less than 70 years of age at time of transplant. Several randomized trials demonstrated the superiority of autologous stem-cell transplant over conventional chemotherapy in terms of the response rate and event-free survival. However, the median duration of response after this procedure does not exceed three years, and almost all patients ultimately relapse.
An Efficient Method for Rapid Establishment of Fibroblast-Like Cells from Horse Subcutaneous Adipose Tissue
M. Quamrul Islam*
Adipose-tissue derived stromal cells (ADSCs) are easily obtainable multipotent mesenchymal stem cells capable of differentiation into various cell lineages making it an attractive cell source for regenerative medicine. ADSC is generally isolated from subcutaneous adipose tissue by enzymatic digestion of biopsies followed by removal of undigested tissues and seeding of the dissociated cells onto plastic. This is an inefficient procedure where only a tiny fraction of cells are established in culture and rest of the cells are lost.